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U.S.
Food & Drug Administration Center for Food Safety & Applied Nutrition Foodborne Pathogenic Microorganisms and Natural Toxins Handbook |
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Entamoeba histolytica
| 1. Name of the Organism:
Entamoeba histolytica |
This is a single celled parasitic animal, i.e., a protozoa, that infects predominantly humans and other primates. Diverse mammals such as dogs and cats can become infected but usually do not shed cysts (the environmental survival form of the organism) with their feces, thus do not contribute significantly to transmission. The active (trophozoite) stage exists only in the host and in fresh feces; cysts survive outside the host in water and soils and on foods, especially under moist conditions on the latter. When swallowed they cause infections by excysting (to the trophozoite stage) in the digestive tract. |
| 2. Nature of Acute Disease: | Amebiasis (or amoebiasis) is the name of the infection caused by E. histolytica. |
| 3. Nature of Disease: | Infections that sometimes last for years may be
accompanied by 1) no symptoms, 2) vague gastrointestinal
distress, 3) dysentery (with blood and mucus). Most
infections occur in the digestive tract but other tissues
may be invaded. Complications include 4) ulcerative and
abscess pain and, rarely, 5) intestinal blockage. Onset
time is highly variable. It is theorized that the absence
of symptoms or their intensity varies with such factors
as 1) strain of amoeba, 2) immune health of the host, and
3) associated bacteria and, perhaps, viruses. The
amoeba's enzymes help it to penetrate and digest human
tissues; it secretes toxic substances. Infectious Dose--Theoretically, the ingestion of one viable cyst can cause an infection. |
| 4. Diagnosis of Human Illness: | Human cases are diagnosed by finding cysts shed with the stool; various flotation or sedimentation procedures have been developed to recover the cysts from fecal matter; stains (including fluorescent antibody) help to visualize the isolated cysts for microscopic examination. Since cysts are not shed constantly, a minimum of 3 stools should be examined. In heavy infections, the motile form (the trophozoite) can be seen in fresh feces. Serological tests exist for long-term infections. It is important to distinguish the E. histolytica cyst from the cysts of nonpathogenic intestinal protozoa by its appearance. |
| 5. Associated Foods: | Amebiasis is transmitted by fecal contamination of drinking water and foods, but also by direct contact with dirty hands or objects as well as by sexual contact. |
| 6. Relative Frequency of Disease: | The infection is "not uncommon" in the tropics and arctics, but also in crowded situations of poor hygiene in temperate-zone urban environments. It is also frequently diagnosed among homosexual men. |
| 7. Course of Disease and Complications: | In the majority of cases, amoebas remain in the gastrointestinal tract of the hosts. Severe ulceration of the gastrointestinal mucosal surfaces occurs in less than 16% of cases. In fewer cases, the parasite invades the soft tissues, most commonly the liver. Only rarely are masses formed (amoebomas) that lead to intestinal obstruction. Fatalities are infrequent. |
| 8. Target Populations: | All people are believed to be susceptible to infection, but individuals with a damaged or undeveloped immunity may suffer more severe forms of the disease. AIDS / ARC patients are very vulnerable. |
| 9. Food Analysis: | E. histolytica cysts may be recovered from contaminated food by methods similar to those used for recovering Giardia lamblia cysts from feces. Filtration is probably the most practical method for recovery from drinking water and liquid foods. E. histolytica cysts must be distinguished from cysts of other parasitic (but nonpathogenic) protozoa and from cysts of free-living protozoa. Recovery procedures are not very accurate; cysts are easily lost or damaged beyond recognition, which leads to many falsely negative results in recovery tests. (See the FDA Bacteriological Analytical Manual.) |
| 10. Selected Outbreaks: | Literature references can be found at the links below. |
| The most dramatic incident in the USA was the Chicago World's Fair outbreak in 1933 caused by contaminated drinking water; defective plumbing permitted sewage to contaminate the drinking water. There were 1,000 cases (with 58 deaths). In recent times, food handlers are suspected of causing many scattered infections, but there has been no single large outbreak. | |
| MMWR 34(9):1985 | In October 1983, the Los Angeles County (California) Department of Health Services was notified by a local medical laboratory of a large increase in the laboratory's diagnoses of intestinal amebiasis (Entamoeba histolytica infection). Thirty-eight cases were identified from August to October. The laboratory staff estimated that, before August, they had diagnosed approximately one E. histolytica infection per month. A preliminary investigation failed to identify a common source of the infection. |
| Morbidity and Mortality Weekly Reports | For more information on recent outbreaks see the CDC. |
| 11. Education and Background Resources: | Literature references can be found at the links below. |
| Loci index for genome Entamoeba histolytica | Available from the GenBank Taxonomy database, which contains the names of all organisms that are represented in the genetic databases with at least one nucleotide or protein sequence. |
| Amebiasis (Entamoeba histolytica) infection FAQ's | What is amebiasis? What are the symptoms of amebiasis? How soon after exposure do symptoms appear? How can I get it? Who is at risk? What should I do if I think I have it? How is it diagnosed and how is it treated? How can it be prevented during travel in developing countries? Should I be concerned about spreading infection to the rest of my household? |
| Nonpathogenic Intestinal Amebae Infection FAQ's | Where do these amebae live in the body? How did they get there? How long do these amebae stay in my body? Should I be treated for these amebae? |
| 12. Molecular Structural Data: | None currently available. |
mow@cfsan.fda.gov
January 1992 with periodic updates
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