Figure 10-3. Microslide gel diffusion test as toxin detection system¹.

¹Antiserum to B. cereus diarrheagenic antigen is in well 12; known reference enterotoxin is in well 3 to produce reference lines; test preparations are in wells 2 and 4. Interpret reactions as follows: (1) No line development between test preparations and antisera—absence of B. cereus toxin; (2) coalescence of test preparation line from well 4 with enterotoxin reference line—presence of enterotoxin in well 4; (3) coalascence of test preparation line from wells 2 and 4 with enterotoxin reference—presence of enterotoxin in wells 2 and 4.