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The hazard develops when a batter mix is exposed to temperatures favorable for S. aureus growth for sufficient time to permit toxin development. S. aureus will grow at temperatures as low as 7ºC (44.6ºF) and at a water activity as low as 0.83. However, toxin formation is not likely at temperatures lower than 10ºC (50°F). For this reason, toxin formation can be controlled by minimizing exposure of hydrated batter mixes to temperatures above 10ºC (50°F). Exposure times greater than 12 h for temperatures between 10ºC (50°F) and 21.1ºC (70°F) could result in toxin formation. Exposure times greater than 3 h for temperatures above 21.1ºC (70°F) could also result in toxin formation (FDA, 1998).
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Dial thermometers
Immerse the stem of the dial thermometer in the ice-water slurry for a minimum of 2 min. After 2 min, read the temperature on the thermometer(s) without removing it (them) from the ice/water slurry. Adjust the thermometer to 0ºC (32ºF), if necessary, by gripping the hex nut under the dial with a wrench or pliers and turning the dial face until the pointer reads the temperature correctly. To check several thermometers for accuracy at the same time, punch holes in a plastic lid and insert the thermometers through the holes and into the ice-water slurry. Paper clips may be formed to hold the thermometers in place. The thermometers should not touch the sides or the bottom of the
container nor should they touch each other. The case around the dial thermometer generally has a metal hoop on the pocket clip that can be used to hold the thermometer in an ice-water slurry.
Electronic thermometers
Immerse the probe of the electronic thermometer in the ice/water slurry for a minimum of 2 min. After 2 min, read the temperatures without removing the probe from the ice/water slurry. Adjust the thermometer to 0ºC (32ºF), if necessary, by following the manufacturer's directions. These thermometers are generally adjusted with a zeroing screw.
Because of the complexity involved in calibrating a thermometer in boiling water, this method should only be used to confirm that a thermometer calibration at 0ºC (32ºF) is measuring accurately at higher temperatures.
Heat the freshwater until a rolling boil is achieved. Immerse the stem of the dial thermometer or the probe of the electronic thermometer in the boiling water for a minimum of 1 min. After at least a min, read the temperatures on the thermometer without removing it from the boiling water. Adjust the thermometer as necessary to the calculated boiling point for the altitude and atmospheric pressure.
ºC = 5/9 ´ (º F - 32)
º F = 32 + º C ´ 9/5
The normal human body temperature is 37ºC (98.6ºF). Freshwater boils at 100ºC (212ºF) at sea level at a standard atmospheric pressure (760 mm Hg), and freezes at 0ºC (32ºF). The freezing point of water does not change with normal variations of atmospheric pressure.
The boiling point of water varies with changes in atmospheric pressure; at higher altitudes the boiling point of water is lowered depending on the altitude. If you are using the boiling point of water to calibrate a thermometer, you must estimate the boiling point is based on your altitude. In general, for each 293 meter (960-foot) increase in altitude the boiling point changes by 1ºC (1.8ºF), however, it is the change in atmospheric pressure that causes the change in the boiling point. Please refer to a handbook of chemistry and physics for the exact boiling point of water at different atmospheric (vapor) pressures. To properly determine the atmospheric pressure you will also need a barometer (De Beer, 1998).
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De Beer, J. 1998. "Accurately measuring seafood temperatures." http://seafood.ucdavis.edu/pubs/tempdoc.htm (16 June, 1998).
FDA. 1998. Staphylococcus aureus toxin formation in hydrated batter mixes. Ch. 15. In Fish and Fishery Products Hazards and Controls Guide, 2nd ed., p. 183-188. Department of Health and Human Services, Public Health Service, Food and Drug Administration, Center for Food Safety and Applied Nutrition, Office of Seafood, Washington, DC.