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LOCUS AGANTIFU 954 bp DNA linear PLN 14-JUN-1994
DEFINITION A.giganteus AFP gene for antifungal protein.
ACCESSION X60771
VERSION X60771.1 GI:2307
KEYWORDS afp gene; antifungal protein.
SOURCE Aspergillus giganteus.
ORGANISM Aspergillus giganteus
Eukaryota; Fungi; Ascomycota; Pezizomycotina; Eurotiomycetes;
Eurotiales; Trichocomaceae; mitosporic Trichocomaceae; Aspergillus.
REFERENCE 1 (bases 1 to 954)
AUTHORS Wnendt,S.
TITLE Direct Submission
JOURNAL Submitted (21-JUN-1991) S. Wnendt, Technische Universitaet Berlin,
Fachgebiet Mikrobiologie und Genetik, Seestr. 13, D-1000 Berlin 65,
FRG
REFERENCE 2 (bases 1 to 954)
AUTHORS Wnendt,S., Ulbrich,N. and Stahl,U.
TITLE Molecular cloning, sequence analysis and expression of the gene
encoding an antifungal-protein from Aspergillus giganteus
JOURNAL Curr. Genet. 25 (6), 519-523 (1994)
MEDLINE 94363778
PUBMED 8082203
COMMENT See X53432 for overlapping sequence.
FEATURES Location/Qualifiers
source 1..954
/organism="Aspergillus giganteus"
/strain="MDH 18894"
/db_xref="taxon:5060"
/cell_type="mycelia"
/clone_lib="lambda EMBL3"
TATA_signal 10..15
gene 150..579
/gene="AFP"
mRNA join(<150..293,383..463,520..>579)
/gene="AFP"
CDS join(150..293,383..463,520..579)
/gene="AFP"
/codon_start=1
/product="antifungal protein precursor"
/protein_id="CAA43181.1"
/db_xref="GI:2308"
/db_xref="SWISS-PROT:P17737"
/translation="MKFVSLASLGFALVAALGAVATPVEADSLTAGGLDARDESAVLA
TYNGKCYKKDNICKYKAQSGKTAICKCYVKKCPRDGAKCEFDSYKGKCYC"
sig_peptide 150..212
/gene="AFP"
mat_peptide join(279..293,383..463,520..576)
/gene="AFP"
exon <150..293
/gene="AFP"
intron 294..382
/gene="AFP"
exon 383..463
/gene="AFP"
intron 464..519
/gene="AFP"
exon 520..>579
/gene="AFP"
polyA_signal 708..713
BASE COUNT 257 a 232 c 220 g 245 t
ORIGIN
1 tcattcgtgt ataaaagggt gtcgtcaacc caggcttttt tgaaaagtat caacatcact
61 atcaataacc accaagacac actctgatct atccaatcca ctacgcaaaa agcaaacaaa
121 acccccgcac ccttctatct caattcatca tgaagttcgt ttctctcgct tctttgggat
181 tcgccctcgt tgctgccctt ggcgcggttg ccacccccgt tgaagccgat tctctcaccg
241 ctggtggtct ggatgcaaga gatgagagcg cggttttggc cacatacaat ggcgtaggtc
301 ttctcttctc agaaatccca ctgcccaggt agtgtctgag cgcgatggat ctcaactaaa
361 cttccttttt ttccgtcccc agaaatgcta caagaaggat aatatctgca agtacaaggc
421 acagagcggc aagactgcca tttgcaagtg ctatgtcaaa aaggtatgta cttctatact
481 gattgaggga aggaagaatg aagtagctga tgactttagt gcccccgcga cggcgcgaaa
541 tgcgagtttg acagctacaa ggggaagtgc tactgctaga cggtgagcga agggacgaag
601 taggctgggg gttattttac tctgctctgc agatcagacg agtcattctt tattgtaaag
661 agacgtgagg agggtattca acataccggc tcatgatcta gccatgaatt aaataagcaa
721 atacataggt ttaccatgtc tacttctcat cacgtagtgt tcacggcttc aatggtccac
781 ctccaacgta ctattgttta gtagagatct ccacgcacta gtcagaagat actgagtcga
841 agcccccgaa tgaggcgaga tcattgcgcc tagatcataa tagccgtgca taagatctgt
901 cggccgttca atgtggttgc ctaacatcct acaaccgagc aagatgtggc gtgt
//
Aug 28 2002 15:52:55